?DABDCDAC2CGenerating a genetically modified human cell line by gene manipulation. ?Analyses of the evolution of genomic Keywords:Human Evolution,Non-coding RNAs,Retrotransposons,Genome Editing,Genomic ImprintingOurresearchesfocusontherolesoftransposableelements,whichwerespecificallyacquiredinthehumanlineage,intheevolutionofhumangeneregulationandgenomicdisorders.UsinggenomeeditingtechnologybyCRISPR/CASsystem,weproducegeneticallymodifiedhumancelltoinvestigatefunctionofhuman-specificretrotransposedsequences.Wealsoanalyzefunctionofnewlydiscoveredhuman-specificlongnon-codingRNAsbyoverexpressionandknockdownexperimentsinvitro.Shunsuke SUZUKIAssociate Professor, Ph.D.Eukaryoticcellscontaincomplexmembranestructures.Therefore,thecellsuseasophisticatedproteintransportsystem.Recently,manypapersreported“non-classicalsecretionpathway”ineukaryote.WefoundN-glycosylationonseveralsignalpeptidedeletedproteinsinyeast.Ourmainprojectisaclarificationofnon-classicalsecretorypathwayinyeast,Saccharomycescerevisiae.AkiraHOSOMIAssistant Professor, Ph.D.Keywords?Proteintransport?Proteindegradation?Yeast?Biochemistry?MolecularbiologylinesStudies on intracellular protein transport and secretion in yeast.(Non-classical secretory pathway)Studies on intracellular protein degradation in yeast.(ER-associated degradation: ERAD)Applied studies of protein transport (secretion) for cancer research.Identification and functional analyses of novel RNA transcripts associated with human-specific retrotransposed gene copies.?Analyses of function and evolutionary impact of novel human-specific long non-coding RNAs.?Analyses of the potential role of human-specific retrotransposons in regulation of the human genome.imprinting in mammals.Each research project is performed by a small research group.Yeast growth assay.Gene deletion mutants shows sensitivity.CImaging data (immunofluorescent staining)Integrated Bioscience DivisionIntegrated Bioscience DivisionLaboratory ofEvolutionary GenomicsLaboratory of Molecular cell microbiology
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