49Department ofDepartment ofOutlook for researchDecellularizedtissueshavesubstance-specificfunctions,andbybindingtocells,theymaybeabletocreatecomplexbiologicaltissuesandorgansinsideandoutsidethebody.Wealsobelievethatwecandevelopmaterialswithunprecedentedcharacteristicsbymakingfulluseoftechnologiesinvariousfields.Outlook for students after graduationThroughresearch,Iwantyoutolearntothink,act,andworkasateam.Ihopethatyouwillmakeuseofyourexperienceinthelaboratoryandplayanactiveroleinvariousfields.Outlook for researchInadditiontocreatingplantsthatdonotneedpesticides,wecancreaterawmaterialsforindustryandpharmaceuticals.Throughtheuseofgeneticallymodifiedplants,thesesubstanceswillbecreatedusingonlysolarenergyandCO2.Outlook for students after graduationWhilemanygraduatesareemployedbyfoodcompanies,othersworkatcomputerandchemicalcompaniesandaspublicofficials,forexampleasplantprotectionofficers.AssociateProfessorNEGISHIJunAfterreceivingadoctorateinTokyoMedicalandDentalUniversity,AssociateprofessorNegishiworkedasaJSPSpostbeforecomingADEKAasaresearchworker.Hisareasofresearcharebiologicalmaterials.AssociateProfessorNOGAWAMasahiroAssociateNogawatookhiscurrentpositionafterworkingasanassistantprofessorattheNagaokaUniversityofTechnologyandasanassistantprofessorFacultyofTextileScienceandTechnologyatHisprincipalareasofresearcharegeneticengineeringandappliedmicrobiologyfocusingonplantsandmicroorganisms.Decellularizedtissueobtainedbyremovingcellsfromlivingtissueconsistsofextracellularmatrix(ECM)andisknowntohavetissue-specificfunctions.Inourlaboratory,wecreateandanalyzedecellularizedtissuefromvarioustissues,clarifythefactorsandcharacteristics,andaimtoapplyittotissueregeneration.Vacuumpressureimpregnationmethod(VPI)usedinmetalprocessingisatechnologythatenablestheefficientintroductionofsolutionsintomaterials.ByapplyingVPItobiomaterialresearch,weaimtodevelopporousmaterialsthatcontaincellsandcompositematerialswithnewproperties.IamcarryingoutresearchonhowtouseA.tumefaciens,whichhastheabilitytoinsertitsownDNAintothechromosomesofplants,todevelopDNAinsertionmethodsfornumerouscrops;researchtoinfectplantswithA.tumefacienstocreateusefulsubstances;andresearchtoalterA.tumefaciensthroughgeneticengineeringtogivethebacteriumnewcapabilities.ProfessorintheUniversity.ShinshuPromotion of neurite outgrowth of decellularized tissues.Wild type VirE2VirE2proteintransfersforeignDNAintoplantnucleus.ModifiedVirE2proteinlocalizedinplastid(chloroplast).Blue:nucleus,Green:modifiedVirE2.NON-transformantTransformedmulberryproducedmorecytokininfromiptgenewhichintegratedintoplantgenomebyinplantamethod.Transformantdidnotshowapicaldominance.Cell introduction into a porous material by VPI.Modified VirE2TransformantApplied BiologyApplied BiologyBiomaterials that take advantage of the characteristics of biological tissuesUtilizing Agrobacterium tumefaciens, which carries out genetic engineering on its own
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