ATissue culture experiments are necessary for understanding at molecular basis. Right picture indicates myotube formation after myogenic stimuli. BNeuronOHOOH0OOHOOHOHOOACDBCHO活性ありTakeshiHOSAKAAssociateProfessor,Ph.D.Secondary metabolite productionMice and Drosophilaas a model for studying about the molecular mechanism of mammary gland development and neurodegeneration.Tissue culture experimentSpore maturationSporulationAerial mycelium formationSporesLife cycle of actinomycetesMammarygland1050Spore germinationSubstrate mycelium formationShinichiYONEKURAProfessor,Ph.D./Skeletalmuscle/Mammarygland/Neurodegenerativedisease/Cellbiology/MolecularBiologyCurrentresearchinourlabfocusesonunderstandinggrowthanddevelopmentatthecellularleveltoidentifymethodstoimproveefficiencyofanimalproduction.Regardingtheanimalproduction,currentlytherearetwomajorfocusesinthelaboratory.Thefirstistounderstandthemolecularmechanismofskeletalmuscledevelopment.Thesecondfocusinthelaboratoryistodeterminethemolecularmechanismofmammaryglanddevelopment.Inaddition,weareinvestigatingthemolecularmechanismofneurodegenerativediseaseandtryingtofindoutthepreventionfactorsforthesediseases.Originally,anantibioticisdefinedasachemicalcompoundthatisproducedbyamicroorganism,suppressesthegrowthofothermicroorganisms,andhaslittletoxicitytoanimals.Speakingofantibiotics,almosteveryonecanimaginethiseffect.Meanwhile,itremainsaprofoundmysterywhatrolesantibioticsplayinthenaturalworld.Withmulti-drugresistantbacteriaemergingoneafteranother,thediscoveryofnovelantibioticsandthedevelopmentofalternativestoantibioticsarebecomingincreasinglycrucial.Thenumberofnewlydiscoveredantibiotics,usuallyisolatedfromsecondarymetabolitesofmicroorganismsincludingactinomycetes,havebeendeclining.Tosolvethispredicament,inadditiontocontinuingthedevelopmentofnovelantibioticsandalternativestoantibiotics,itwouldbeofutmostimportanttounderstandthefundamentalworkingsofantibioticsthemselves,antibiotic-producingmicroorganism,andantibioticresistance.Actinomycetes, Antibiotic, Secondary metabolism, Ribosome engineering, Antibiotic hormesisActinomycetesproduceabundantamountsofnumerousincludingantibiotics,immunosuppressants,andantitumoragents.Whole-genomesequencingstudiesofactinomyceteshaveshownthateachspeciescouldproducemanymoresecondarymetabolitesthanwereexpected.Thisindicatesthatthevastmajorityofremainunexpressedorbarelyexpressedunderstandardlaboratoryconditions.Thus,thereisconsiderableinterestinexploringpracticalmeanstoinducethisgeneticpotentialinactinomycetes,whichcouldresultinisolationofnovelbioactivemetabolites.Ourlaboratoryaimstounderstandthemolecularmechanismsactivatingsecondarymetabolismandtoestablisheffectivemethodsfordevelopingcrypticgeneclustersforsecondarymetabolicpathwaysinmicroorganisms.Molecular mechanisms of skeletal muscle developmentMolecular mechanisms of mammary gland developmentMolecular mechanisms of neurodegenerative disease and explore the prevention factorsbioactivemetabolites,secondarymetabolitesConcentration-dependent effects of lincomycin on secondary metabolism in S. coelciolor A3(2)Ourlatestfindingsdemonstratethatribosome-targetingantibioticsnotonlyfunctionsasanantimicrobialbutalsocanactasanactivatorforsecondarymetabolismofactinomycetes.Giventhisphysiologicalfact,ourlaboratoryhasbeenanalyzingtheconcentration-dependentactivitiesofantibioticsatthemolecularlevelwiththehopeofuncoveringmysteriessuchasmechanismsforsecondarymetabolisminactinomycetesandtherolesofantibioticsinthenaturalworld.29（写真一枚or複数枚組み合わせ）Day 4Day 0104to107CFU ofactinomycetesin1 gram soil（in most soils）Laboratory of Applied and Molecular MicrobiologyBiotechnology Division Biotechnology DivisionLaboratory of Animal Physiologyin vivo experiment (Mice, Drosophila)Lincomycin(?g/mL)
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