?CDABDCCDAC2?Analyses of function and evolutionary ?Analyses of the potential role of human-Laboratory ofEvolutionary GenomicsIdentification and functional analyses of novel RNA transcripts associated with human-specific retrotransposed gene copies.impact of novel human-specific long non-coding RNAs.specific retrotransposons in regulation of the human genome.?Analyses of the evolution of genomic imprinting in mammals.Laboratory of Molecular cell microbiologyKeywords:Human Evolution,Non-coding RNAs,Retrotransposons,Genome Editing,Genomic ImprintingKeywords?Proteintransport?Proteindegradation?Yeast?Biochemistry?MolecularbiologyOurresearchesfocusontherolesoftransposableelements,whichwerespecificallyacquiredinthehumanlineage,intheevolutionofhumangeneregulationandgenomicdisorders.UsinggenomeeditingtechnologybyCRISPR/CASsystem,weproducegeneticallymodifiedhumancelltoinvestigatefunctionofhuman-specificretrotransposedsequences.Wealsoanalyzefunctionofnewlydiscoveredhuman-specificlongnon-codingRNAsbyoverexpressionandknockdownexperimentsinvitro.Shunsuke SUZUKIAssociate Professor, Ph.D.AkiraHOSOMIAssistant Professor, Ph.D.linesIntegrated Bioscience DivisionEukaryoticcellscontaincomplexmembranestructures.Therefore,thecellsuseasophisticatedproteintransportsystem.Recently,manypapersreported“non-classicalsecretionpathway”ineukaryote.WefoundN-glycosylationonseveralsignalpeptidedeletedproteinsinyeast.Ourmainprojectisaclarificationofnon-classicalsecretorypathwayinyeast,Saccharomycescerevisiae.Integrated Bioscience DivisionStudies on intracellular protein transport and secretion in yeast.(Non-classical secretory pathway)Studies on intracellular protein degradation in yeast.(ER-associated degradation: ERAD)Applied studies of protein transport (secretion) for cancer research.Generating a genetically modified human cell line by gene manipulation. Each research project is performed by a small research group.Yeast growth assay.Gene deletion mutants shows sensitivity.Imaging data (immunofluorescent staining)
元のページ ../index.html#7