ProfessorHOCHIShinichiProfessorHochitookhiscurrentpositionin2008afterworkingasaresearcher(1986to1992)atSnowBrandMilkProductsCo.Ltd.,asavisitingassistantprofessor(1992to1995)andvisitingassociateprofessor(1995to1996)atObihiroUniversityofAgricultureandVeterinaryMedicine,andasanassistantprofessor(1996to1999)andassociateprofessor(1999to2008)intheFacultyofTextileScienceandTechnologyatShinshuUniversity.Heisengagedinresearchphysiology,cryobiology,developmentalengineeringinsmallexperimentalrodents,largedomesticanimals,andhumans.ProfessorHORIETomoakiProfessorHorietookhiscurrentpositionin2010afterworkingasaresearcherattheUniversityofCalifornia,SanDiego,andasanassistantprofessoronspecialcontractwiththeInstituteofPlantScienceatOkayamaUniversity,andthenasanassociateprofessoratShinshuUniversity.Hisresearchcentersonthebasicmolecularphysiologyofplantsandplantgeneticandcellularengineeringbasedonit.reproductiveonandResourcesandOutlook for researchreproductiveOutlook for students after graduationGraduatesareemployedbypharmaceuticalandfood-relatedcompaniesaswellasnationalandlocalorganizations(aspublicofficials).Inwhathasbecomearecenttrend,someskilledgraduatesworkatpotentialembryologists.infertilityhumanOutlook for researchIexpectthatmylab’sresearchcouldbecomeanimportantelementinavoidingfoodandenergyshortagesthatareexpectedinthenearfutureifwecanapplyknowledgeobtainedfrombasicplantsciencetobreedsalt-tolerantplants.Outlook for students after graduationManyofthestudentsinmylabchoosetopursuecareerswithcompanieswhoseworkinvolvesplants.Graduatesalsotendtobeinterestedinworkingforthatdevelopdistinctiveenvironmentaltechnologiesorinbecomingteachers.suchtechnologiesclinicsascompanies43asAdvancedintracytoplasmicsperminjectionandsomaticcellnucleartransplantationmadeitpossibletobringthemammothbacktolife.Furthermore,wehaverecentlyestablishedpluripotentstemcelllinesinlaboratoryrats.IftheseESoriPScellscouldbedifferentiatedintofunctionalgametes,theprocesswouldbetheultimatetreatmentforinfertility.Micromanipulationenablesustohandlenotonlyoocyteswithdiametersof100to120μm,butalsomuchsmallerspermcells.Intracytoplasmicinjectionoffishhook-shapedratspermheadswasdifficulttoachieve,butseveralclueshelpedusexpanditsapplicationtofreeze-driedspermatozoa.MylabfocusesonriceandplantsinthegeniusArabidopsisformolecularphysiologicalstudiesusingwild-typeandNa+transporter-mutantplants.Thepictureshowsanexampleofahydroponiccultureofriceplants.WeanalyzethetargetNa+transporterthatisessentialforplantsalttoleranceintheoocyteexpressionsystemofXenopuslaevis.WeareattemptingtoproducemutagenizedNa+transporters,whichweexpecttoincreasethesalttoleranceofthehostplants.AmicrotubulenetworkcanbefromcentrosomeofspermatozoonimmediatelyafterfertilizationassembledWild type hkt1;1mutant Inmylabwestudyphysiologicalaspectsofearlyeventsduringfertilizationinmammalsaswellastechnicalinnovationsinvolvingthepreservationandutilizationofgeneticresources.MystudentsandIhavepublishedmorethan100researchpaperssofardescribingourresearchintohaploidgametes(spermatozoaandoocytes)anddiploidembryosfromvariousmammalianspecies,includingmice,rats,rabbits,cats,horses,buffalo,cattle,whales,andhumans.Iinvestigatethemolecularmechanismsbymeansofwhichplantsprotectthemselvesfromsalinitystress(high-saltenvironments)usingtheexperimentalmethodsofmolecularbiology,moleculargenetics,andplantphysiology.Ihopetodevelopinnovativetechnologytogeneratesalt-resistantcropsinordertoincreaseagriculturalyieldsinsalt-affectedareas.Applied BiologyApplied BiologyDepartment ofDepartment ofUsing micromanipulation to study the mechanism of fertilization and preserve, regenerate, and utilize genetic resourcesCreating new plants based on knowledge of basic plant science in order to contribute to the development of a sustainable society
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